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| 产品名称： | pTHH37 RW-23 |
|---|---|
| 商品货号： | TS137345 |
| Designations： | pTHH37 RW-23 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C. |
| Vector： | Construct size (kb): 5.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy D14S16 Alleles: A1, A2, A3 |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--4.2, 0.98; BamHI--5.0; AvaI--3.1, 2.0. PstI detects a 3 allele polymorphism with bands between 2-3 kb. MspI and TaqI detect the same polymorphism. Codominant segregation has been shown in 60 families. RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C. Enzyme(s) not detecting polymorphism: RsaI. |
| References： | Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH37) on chromosome 14 D14S16. Nucleic Acids Res. 16: 3593, 1988. PubMed: 2897662 Peter J OConnell, personal communication |