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| 产品名称： | pmIL1AcDNA murine IL-1alpha cDNA |
|---|---|
| 商品货号： | TS137350 |
| Designations： | pmIL1AcDNA murine IL-1alpha cDNA |
| GenBank Number： | X01450 |
| Species： | Mus musculus, mouse |
| Depositors： | U Gubler |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: P388D1 cell line Gene product: interleukin 1, alpha Il1a |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (nt positions according to the published sequence): HpaI--1188; PstI--373; PvuII--789; ScaI--1111; XbaI--1585. Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.0, 2.0; PstI--5.0; PvuII--2.7, 1.5, 0.8; EcoRI--uncut; XbaI--4.7, 0.2. The BstNI site at nt 8 was filled in and a HindIII linker was added. The 3 end of the insert includes the poly(A) tail, the added GC tail, and the BamHI site engineered from the original pMG-5 vector. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Lomedico PT, et al. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature 312: 458-462, 1984. PubMed: 6209582 Ueli Gubler, personal communication |