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| 产品名称： | pI3 |
|---|---|
| 商品货号： | TS137408 |
| Designations： | pI3 |
| Depositors： | KW Minton, C Masters |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 16.0000000000000000 Vector: pI3 (plasmid) Construction: pKK232-8, pUE10 Marker(s):ampR,cmlR Construct size (kb): 16.0 Features: marker(s): ampR, cmlR replicon: pMB1, pUE10 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--17.0; EcoRI--14.0, 4.3; HindIII--17.0. Shuttle vector using ampR selection in E. coli and cmlR selection in Deinococcus radiodurans. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - pUE10 replicon - pUE10 promoter -> - cmlR -> - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940 |
| Shipped： | freeze-dried |