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微信小章| 产品名称： | cos15 |
|---|---|
| 商品货号： | TS137426 |
| Designations： | cos15 |
| GenBank Number： | U07563 |
| Species： | Homo sapiens, human |
| Depositors： | S Clifton, BA Roe |
| Vector： | Construct size (kb): 37.82300186157227 DESCRIPTION OF VECTOR COMPONENT: Name of vector: svPHEP Intact vector size: 9.201 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI ClaI Polylinker sites: HindIII ClaI EcoRI BamHI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L19900 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ABL1 Other gene symbols: ABL Genomic copy number: unique Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1 Contains complete coding sequence?: N Chromosome: 9; Localization: 9 q34.1 Tissue: lung carcinoma Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 28.622 Cross references: DNA Seq. Acc.: U07563 Nucleotides 1-29622 of the insert correspond to nucleotides 44062-73683 of U07563. Insert lengths(kb): 28.62199974060059 Tissue: lung carcinoma Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 ABL Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1 |
| Insert Size (kb)： | 28.622 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--15.5, 12.5, 7.7, 3.6, 0.65; BglII--8.0, 7.7, 7.0, 4.6, 4.4, 3.1, 2.55, 2.3, 1.75, 1.5, 0.68; EcoRI--7.7 (doublet), 6.2, 3.4 (doublet), 3.1, 2.65, 2.3, 1.85, 1.15, 0.84; HindIII--9.2, 7.7, 5.7, 4.6, 3.3, 3.0, 2.65, 1.8, 1.3, 0.98; NotI-->23.0; SstI--8.0, 7.7, 4.1 (doublet), 3.4, 3.1, 2.4, 2.3, 2.0, 1.55, 1.15. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 1a and extends into intron 8. |
| References： | Groffen J, et al. Isolation of human oncogene sequences (v-fes homolog) from a cosmid library. Science 216: 1136-1138, 1982. PubMed: 6281890 Heisterkamp N, et al. The human v-abl cellular homologue. J. Mol. Appl. Genet. 2: 57-68, 1983. PubMed: 6302194 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |