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| 产品名称： | 28S.3 |
|---|---|
| 商品货号： | TS137435 |
| Organism： | Ictalurus punctatus, channel catfish |
| Tissue： | peripheral blood |
| Cell Type： | lymphocyte T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The cells constitutively produce a supernatant factor(s) with growth promoting activity PubMed: 9809582. The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Derivation： | The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
The cells are immunoglobulin negative PubMed: 9809582.
The cells constitutively produce a supernatant factor(s) with growth promoting activity PubMed: 9809582. |
| Comments： | The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
The cells are immunoglobulin negative PubMed: 9809582.
The cells constitutively produce a supernatant factor(s) with growth promoting activity PubMed: 9809582. |
| Complete Growth Medium： | A 1:1 mixture of Leibovitzs L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 27.0°C |
| Name of Depositor： | NW Miller |
| Year of Origin： | 1996 |
| References： | Wilson MR, et al. T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582 |