产品名称: | pCEA1 |
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商品货号: | TS137444 |
Designations: | pCEA1 |
Species: | Homo sapiens, human |
Vector: | Construct size (kb): 5.599999904632568 |
Insert: | DNA: cDNA Insert lengths(kb): 0.8299999833106995 Tissue: colon adenocarcinoma Gene product: carcinoembryonic antigen CEA |
Insert Size (kb): | 0.830 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
Comments: | Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.54 + others; EcoRI--5.3; HindIII--5.3; BamHI--5.3; SalI--5.3. The insert contains HinfI, HpaII, PstI, HincII, SstI, and PstI sites. The PstI sites at the ends were created by the cloning procedure. This is a 5 end probe for CEA. Open reading frame encoded in pCEA1 and pCEA2 contains 2 closely-related repeats of 178 amino acids each. pCEA1 contains repeat a and part of repeat b; pCEA2 contains repeat b and part of repeat a. The 0.534 kb PstI fragment detects mRNA of 3.8 and 3.2 kb from mouse mammary tumors. |
References: | Zimmermann W, et al. Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure. Proc. Natl. Acad. Sci. USA 84: 2960-2964, 1987. PubMed: 3033671 |