微信小陈
微信小章| 产品名称： | LL06NS01 |
|---|---|
| 商品货号： | TS137478 |
| Designations： | LL06NS01 |
| GenBank Definition： | H.sapiens SOD-2 gene for manganese superoxide dismutase. |
| GenBank Number： | X65965 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | hamster (CHO)/human lymphocyte UV20HL15-33 hybrid |
| Applications： | By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes. |
| Vector： | Tissue: hamster (CHO)/human lymphocyte UV20HL15-33 hybrid Chromosome: 6 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 28000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The #6 peak overlaps hamster background; narrow window. By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes. About 77% of the library is human (and 23% hamster) by plaque hybridization. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 27. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |