微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUR288-126K/183K |
|---|---|
| 商品货号： | TS137489 |
| Designations： | pUR288-126K/183K |
| Species： | Tobacco mosaic virus |
| Depositors： | WO Dawson |
| Applications： | produces protein 126K protein produces protein 183K protein |
| Vector： | Construct size (kb): 5.940000057220459 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7400000095367432 Gene product: 126K protein Target Gene: 126K protein, 183K protein |
| Insert Size (kb)： | 0.740 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; HindIII--6.0; EcoRI--5.2, 0.76; BamHI/XbaI--5.2, 0.74. This clone expresses an antigen for the 126K and 183K proteins of tobacco mosaic virus. The internal SmaI-XbaI 126K gene of pTMV204 (nt 259-1000) was ligated to BamHI linkers at the SmaI end and then ligated into BamHI-XbaI digested pUR288. The correct reading frame was confirmed by sequencing. |
| Classification： | Tobamovirus |
| References： | Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986. Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644 William O Dawson, personal communication |