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| 产品名称： | pHAF7 |
|---|---|
| 商品货号： | TS137497 |
| Designations： | pHAF7 |
| GenBank Number： | J00077 |
| Species： | Homo sapiens, human |
| Depositors： | T Tamaoki |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: embryonal carcinoma Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) AFP Alleles: B1, B2, C1, C2, D1, D2 |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.4 + smaller; HindIII--5.7; EcoRI--5.7; AvaI--5.7; BamHI--5.7. Insert includes 5 portion of mRNA except the first 55 nucleotides. Encoded are part of the signal peptide, all of domain 1, and part of domain 2 of the protein. This probe overlaps with pHAF6 (p01088). Enzyme(s) not detecting polymorphism: BamHI, BclI, BglI, BglII, EcoRI, EcoRV, HaeIII, HgiAI, HincII, HindIII, HinfI, KpnI, MspI, MstII, PvuII, RsaI, SacI, Sau3A, StuI, TaqI, XbaI, XmnI using a panel of 10 unrelated Caucasoids. |
| References： | Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439 Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5 to 3 orientation. Cytogenet. Cell Genet. 40: 708, 1985. Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731 |