产品名称: | c9780 |
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商品货号: | TS137528 |
Designations: | c9780 |
GenBank Number: | U10555 |
Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
Depositors: | L Riles, MV Olson |
Vector: | Construct size (kb): 46.29999923706055 |
Insert: | DNA: genomic Insert lengths(kb): 39.84400177001953 Gene product: centromere, chromosome VIII CEN8 |
Insert Size (kb): | 39.844 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.211, 6.549 (vector), 5.352, 4.859, 3.774, 2.022, 1.766, 1.666, 1.335, 1.331, 1.048, 0.940, 0.830, 0.790, 0.790, 0.726, 0.625, 0.590, 0.589, 0.545, 0.417, 0.391, 0.380 (vector). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 108805-109878 of the sequence of chromosome VIII. |
Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
References: | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |