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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS137569 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | ET12567 |
| Application： | It is the key host strain for streptomyces workers worldwide.
This is a host for isolation of non-methylated DNA for transfer to other species. This strain is used as a host to isolate DNA and plasmids for transformation into Streptomyces or any other organism that has a methylation-sensitive restriction system. |
| Isolation： | Derived from existing strain, constructed from strain GM2929 by T. MacNeil |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 |
| Type Strain： | no |
| Medium： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | DJ MacNeil, T MacNeil |
| Special Collection： | NSF - Bacteriology |
| References： | MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955 Douglas J MacNeil, personal communication |