微信小陈
微信小章| 产品名称： | Cryptococcus neoformans (Sanfelice) Vuillemin |
|---|---|
| 商品货号： | TS137578 |
| Strain Designations： | 52 52D, CDC B3179 |
| Alternate State： | Filobasidiella neoformans Kwon-Chung |
| Application： | Biomedical Research and Development Material Emerging infectious disease research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Antigenic Properties： | serotype D |
| Preceptrol®： | no |
| Comments： | Phenotypic switching
Antibody response to capsular glucuronoxylomannan, GXM, in mice
Experimental infection in rats
Facultative intracellular pathogen
Crystal formation
Immunoglobulin M specificity to epitopes
Induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta in human microglia
Interaction between lung surfactants and alveolar macrophages in defense against Cryptococcus neoformans
Melanization decreases the suseptibility of Cryptococcus neoformans to enzymatic degradation.
Multilocus enzyme typing |
| Medium： | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC |
| Name of Depositor： | JE Bennett |
| Isolation： | Human cerebrospinal fluid, Maryland, USA |
| Cross References： | Nucleotide (GenBank) : L05428 17S rRNA gene, complete gene Nucleotide (GenBank) : L14068 5.8S and 25S ribosomal RNA sequences Nucleotide (GenBank) : L27078 5S ribosomal RNA and intergenic spacer Nucleotide (GenBank) : AF032431 orotate phosphoribosyltransferase (URA5) gene, complete coding sequence |
| References： | Wilson DE, et al. Serologic grouping of Cryptococcus neoformans. Proc. Soc. Exp. Biol. Med. 127: 820-823, 1968. PubMed: 5651140 Fan M, et al. The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans. J. Med. Vet. Mycol. 33: 215-221, 1995. PubMed: 8531018 Brandt ME, et al. Multilocus enzyme typing of Cryptococcus neoformans. J. Clin. Microbiol. 31: 2819-2823, 1993. PubMed: 8253996 Gyetko MR, et al. Urokinase is required for the pulmonary inflammatory response to Cryptococcus neoformans. J. Clin. Invest. 97: 1818-1826, 1996. PubMed: 8621764 Mukherjee S, et al. J774 murine macrophage-like cell interactions with Cryptococcus neoformans in the presence and absence of opsonins. J. Infect. Dis. 173: 1222-1231, 1996. PubMed: 8627076 Wang Y, et al. Melanin, melanin "ghosts," and melanin composition in Cryptococcus neoformans. Infect. Immun. 64: 2420-2424, 1996. PubMed: 8698461 Gross NT, et al. In vitro effect of lung surfactant on alveolar macrophage defence mechanisms against Cryptococcus neoformans. Mycopathologia 144: 21-27, 1999. PubMed: 10422270 Nussbaum G, et al. Molecular and idiotypic analyses of the antibody response to Cryptococcus neoformans glucuronoxylomannan-protein conjugate vaccine in autoimmune and nonautoimmune mice. Infect. Immun. 67: 4469-4476, 1999. PubMed: 10456888 Fries BC, et al. Phenotypic switching in Cryptococcus neoformans results in changes in cellular morphology and glucuronoxylomannan structure. Infect. Immun. 67: 6076-6083, 1999. PubMed: 10531269 Goldman DL, et al. Persistent Cryptococcus neoformans pulmonary infection in the rat is associated with intracellular parasitism, decreased inducible nitric oxide synthase expression, and altered antibody responsiveness to cryptococcal polysaccharide. Infect. Immun. 68: 832-838, 2000. PubMed: 10639453 Feldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240 Feldmesser M, et al. Intracellular crystal formation as a mechanism of cytotoxicity in murine pulmonary Cryptococcus neoformans infection. Infect. Immun. 69: 2723-2727, 2001. PubMed: 11254641 Nakouzi A, et al. Molecular basis for immunoglobulin M specificity to epitopes in Cryptococcus neoformans polysaccharide that elicit protective and nonprotective antibodies. Infect. Immun. 69: 3398-3409, 2001. PubMed: 11292763 Goldman D, et al. Cryptococcus neoformans induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta in human microglia: role of specific antibody and soluble capsular polysaccharide. Infect. Immun. 69: 1808-1815, 2001. PubMed: 11179358 Olszewski MA, et al. Regulatory effects of macrophage inflammatory protein 1alpha/CCL3 on the development of immunity to Cryptococcus neoformans depend on expression of early inflammatory cytokines. Infect. Immun. 69: 6256-6263, 2001. PubMed: 11553568 Beenhouwer DO, et al. Both Th1 and Th2 cytokines affect the ability of monoclonal antibodies to protect mice against Cryptococcus neoformans. Infect. Immun. 69: 6445-6455, 2001. PubMed: 11553589 Rosas AL, Casadevall A. Melanization decreases the suseptibility of Cryptococcus neoformans to enzymatic degradation. Mycopathologia 151: 53-56, 2001. |