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| 产品名称： | BL29-56 SGC35351, U20582 |
|---|---|
| 商品货号： | TS137603 |
| Designations： | BL29-56 SGC35351, U20582 |
| GenBank Number： | U20582 |
| Species： | Homo sapiens, human |
| Depositors： | R Sanders |
| Vector： | Construct size (kb): 4.669000148773193 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ACTL* Gene name: actin-like Contains complete coding sequence?: N Chromosome: 12 Genome: Homo sapiens Gene symbol: ACTL* Gene name: actin-like Contains complete coding sequence?: N Chromosome: 16 Genome: Homo sapiens Gene symbol: ACTL* Gene name: actin-like Contains complete coding sequence?: N Chromosome: 1; Localization: 1, 69.85 cR Tissue: BL29 cell line Disease: Burkitts lymphoma Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 1.769 Cross references: DNA Seq. Acc.: U20582; EST: EST056 Insert lengths(kb): 1.769000053405762 Tissue: BL29 cell line Burkitts lymphoma Disease: Burkitts lymphoma Gene product: actin-like ACTL* |
| Insert Size (kb)： | 1.769 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.35, 0.45; BamHI--4.6; HindIII--4.6; PstI--3.5, 1.0, 0.2, 0.1; SmaI--3.8, 0.8. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.51, 1.68; PvuII--0.53, 0.72; PstI--0.53, 1.45, 1.69; EcoRI--1.35. |