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微信小章| 产品名称： | pcDmouseIL-2 MT-1 |
|---|---|
| 商品货号： | TS137636 |
| Designations： | pcDmouseIL-2 MT-1 |
| GenBank Number： | K02292 |
| Species： | Mus musculus, mouse |
| Depositors： | DNAX Research Institute of Molecular and Cellular Biology, Inc., EP Ching, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications： | in another host, produces protein interleukin 2 |
| Vector： | Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDV1 Intact vector size: 3.000 Type of vector: plasmid Cloning sites: SalI ClaI HindIII EcoRI KpnI Polylinker sites: Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 late Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: mouse Gene symbol: Il2 Genomic copy number: unique Gene name: interleukin 2 Contains complete coding sequence?: Y Chromosome: 3; Localization: 3 Strain: C57BL/6 Tissue: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced Type of DNA: cDNA Insert 5 end: Modification: GC Insert 3 end: Modification: AT Insert size (kb): 0.816 Cross references: DNA Seq. Acc.: K02292 Insert lengths(kb): 0.8159999847412109 Tissue: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced Gene product: interleukin 2 Il2 Target Gene: interleukin 2 |
| Insert Size (kb)： | 0.816 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 0.94; PstI--2.9, 1.35; XhoI--3.0, 1.1. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.70; BglII--0.31; HindIII--0.38; PstI--0.04; PvuII--0.20; SacI--0.22; SphI--0.06. Contains the complete coding sequence (nt 49-557). Digestion with XhoI excises the insert plus flanking vector sequence. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Yokota T, et al. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc. Natl. Acad. Sci. USA 82: 68-72, 1985. PubMed: 3918306 Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742 Lee FD, et al. cDNA clones coding for polypeptides exhibiting murine interleukin-2 activity. US Patent 4,798,789 dated Jan 17 1989 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |