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| 产品名称： | pJCF62 |
|---|---|
| 商品货号： | TS137665 |
| Designations： | pJCF62 |
| Depositors： | JG Cobley |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.1000003814697300 Vector: pJCF62 (plasmid) Construction: pJCF22, pFDA ori Marker(s):cmlR,kanR Construct size (kb): 12.10000038146973 Features: marker(s): kanR, cmlR replicon: pMB1, pFDA |
| Applications： | contains sequence DNA Segment shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.55, 2.4, 2.2 (doublet), 2.15, 1.1; BamHI--uncut; ClaI--12.1, ClaI/BamHI--12.1. Can be mobilized into F. diplosiphon using the conjugative plasmid RP4. Shuttle vector. A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid. Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the AsuII site of pJCF22. The order of the major features of the plasmid is: kanR - cmlR - F. diplosiphon pFDA ori - bom - oriV. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056 Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495 John G Cobley, personal communication |
| Shipped： | freeze-dried |