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| 产品名称： | pBJ5-HD1-F |
|---|---|
| 商品货号： | TS137674 |
| Designations： | pBJ5-HD1-F |
| GenBank Definition： | Human histone deacetylase HD1 mRNA, complete cds |
| GenBank Number： | U50079 |
| Species： | Homo sapiens, human |
| Depositors： | PG Mark |
| Applications： | In another host, produces protein histone deacetylase 1 |
| Vector： | Name of construct: pBJ5-HD1-F Excise insert: BamHI Promoter giving sense strand: SRalpha Size of construct (kb): 4.8 Markers: ampR Name of vector: pBJ5 Intact vector size: 3.300 Type of vector: plasmid Vector end: SacII Vector end: EcoRI Cloning sites: NotI SacII SfiI EcoRI Polylinker sites: NotI SacII SfiI EcoRI BamHI Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): promoter for expression: SRalpha, → other: 16S splice site, → MCS: NotI...EcoRI, → transcription terminator: polyadenylation, → marker(s): ampR |
| Insert： | Genome:xa0Human
Gene symbol: HDAC1
Gene name: histone deacetylase 1 Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 p34.1 Tissue: Jurkat T cells Type of DNA: cDNA Insert 5 end: NotI, SacII Insert 3 end: SalI Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: U50079 Nucleotides ~1-1449 of the insert correspond toxa0nucleotides 111-1559 of U50079. Insert lengths(kb): 1.5
Tissue: Jurkat T cells
Gene product: histone deacetylase 1 HDAC1
Target Gene: histone deacetylase 1 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.3, 1.5; EcoRV--4.8. Produces protein tagged at the C-terminus with the FLAG epitope. |
| References： | Taunton J, et al. A Mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272: 408-411, 1996. PubMed: 8602529 |