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| 产品名称： | pYNB3.1 |
|---|---|
| 商品货号： | TS137693 |
| Designations： | pYNB3.1 |
| Species： | Homo sapiens, human |
| Depositors： | RL White |
| Vector： | Construct size (kb): 6.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: HincII Vector end: HincII Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D7S372 Genomic copy number: unique Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 7; Localization: 7 q35-qter Type of DNA: genomic Insert end: RsaI Insert end: RsaI Insert size (kb): 4.0 Cross references: Insert lengths(kb): 4.0 Gene product: DNA Segment, single copy D7S372 Alleles: R1, R2 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 2.7, 1.3; BamHI--3.0, 1.9, 1.9; EcoRI--6.7; HindIII--5.5, 1.3; PstI--2.8, 1.5, 1.3, 0.8, 0.4. Enzymes(s) not detecting polymorphism: MspI, TaqI. |
| References： | Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220 Raymond L White, personal communication |