微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRS425 |
|---|---|
| 商品货号： | TS137804 |
| Designations： | pRS425 |
| Depositors： | TW Christianson |
| Biosafety Level： | 1 |
| Host： | Shipped in E. coli XL-1 Blue |
| Vector Information： | Size (kb): 6.849 Vector: pRS425 (phagemid) Promoters: Promoter T7 (phi10) Construction: pJS92, pRS305, pBluescript II SK+ Marker(s):LEU2,ampR Construct size (kb): 6.849 Features: insert detection: lacZ marker(s): LEU2 marker(s): ampR promoter: T3 promoter: T7 (phi10) promoter: lac replicon: 2 micron replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.8; EcoRI--4.8, 2.1; XbaI--3.6, 3.2; PvuII--6.4, 0.5. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.6 + or - 0.6% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - LEU2. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568 |
| Related Products： | component of:ATCC 77108 component of:ATCC 87533 component of:ATCC 87535 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli XL-1 Blue containing the phagemid. |