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微信小章| 产品名称： | yHPRT D12A5, yWXD931 |
|---|---|
| 商品货号： | TS137810 |
| Designations： | yHPRT D12A5, yWXD931 |
| Species： | Homo sapiens, human |
| Depositors： | C Huxley |
| Applications： | in another host, produces protein hypoxanthine phosphoribosyltransferase |
| Vector： | Construct size (kb): 0.0 |
| Insert： | DNA: genomic Tissue: lymphoblastoid CGM1 cell line Gene product: hypoxanthine phosphoribosyltransferase HPRT Alleles: A1, A2, A3 Target Gene: hypoxanthine phosphoribosyltransferase |
| Media： | ATCC® Medium 1798: aAHC medium for YAC clones |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb in order from the CEN end): NruI--100, 130, 40, 410; MluI--230, 105, 100, 245. A complete cDNA probe detects a 70 kb BssHII fragment in both YAC and human DNA. The gene is located near the center of the insert, with the 5 end toward the yeast centromere. It is contained in a 100 kb MluI fragment. When transferred to mouse L-cell line A-9 (GM00346B, HPRT-negative), the YAC conferred the ability to grow in HAT medium. |
| References： | Gnirke A, Huxley C. Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNA. Somatic Cell Mol. Genet. 17: 573-580, 1991. PubMed: 1767336 Imai T, Olson MV. Second-generation approach to the construction of yeast artificial-chromosome libraries. Genomics 8: 297-303, 1990. PubMed: 2249850 Huxley C, et al. The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. Genomics 9: 742-750, 1991. PubMed: 2037299 |