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微信小章| 产品名称： | Blastocrithidia culicis (Novy et al.) Wallace and Johnson |
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| 商品货号： | TS137817 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | mosquito, Aedes vexans, 1942 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Endosymbiont-bearing Nutrition significance of symbiotic bacteria monophyly of endosymbiont-containing trypanosomatids Effects of methylglyoxal bis (guanylhydrazone) on growth Ultrastructure of symbiotic bacteria RNA editing Cell-to-cell interactions Trypomastigotes in cultures Cyclopropane fatty acid |
| Medium： | ATCC® Medium 832: Gracex92s insect medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | KP Chang |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1942 |
| References： | Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371 Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567 Chang KP, Trager W. Nutritional significance of symbiotic bacteria in two species of hemoflagellates. Science 183: 531-532, 1974. PubMed: 4203488 Maslov DA, et al. Evolution of RNA editing in kinetoplastid protozoa: pan-editing preceded 5-editing. Nature 368: 345-348, 1994. PubMed: 8127370 Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 Sousa MA. Cell-to-cell interactions suggesting a sexual process in Herpetomonas megaseliae (Kinetoplastida: Trypanosomatidae). Parasitol. Res. 80: 112-116, 1994. PubMed: 8202449 de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806 Hollar J, et al. Monophyly of endosymbiont containing trypanosomatids: Phylogeny versus taxonomy. J. Eukaryot. Microbiol. 45: 293-297, 1998. PubMed: 9627990 Du Y, et al. Monophyletic origin of beta-division proteobacterial endosymbionts and their coevolution with insect trypanosomatid protozoa. Proc. Natl. Acad. Sci. USA 91: 8437-8441, 1994. PubMed: 7521530 |
| Cross References： | Nucleotide (GenBank) : L29266 ribosomal RNA sequence Nucleotide (GenBank) : L29265 Blastocrithidia culicis ribosomal RNA. Nucleotide (GenBank) : U05817 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial pre-RNA-edited DNA sequence Nucleotide (GenBank) : U05813 kinetoplast (MURF1) and apocytochrome B (cytB) genes, partial coding sequence, and ATPase subunit 6 (MURF4) gene, complete pre-RNA-edited DNA sequence Nucleotide (GenBank) : U05814 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial coding sequence, and cytochrome c oxidase subunit III (COIII) gene, complete coding sequence |