微信小陈
微信小章| 产品名称： | Cochliobolus sativus (Ito et Kuribayashi) Drechsler ex Dastur |
|---|---|
| 商品货号： | TS137868 |
| Strain Designations： | ND90Pr |
| Alternate State： | Bipolaris sorokiniana (Saccardo) Shoemaker, anamorph |
| Application： | Tester for mating type A |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | pathotype 2 |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Mating Type： | MAT-2 (or A) |
| Comments： | differential virulence Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA). |
| Morphology： | After 5 days at 20°C colonies floccose with grey aerial mycelium and reverse dark brown to black.xa0 Later conidia curved to straight, fusiform to broadly ellipsoidal, 3-12 distoseptate, 40-120 x 17-28 µm. |
| Medium： | ATCC® Medium 350: Emerson YpSs agar ATCC® Medium 336: Potato dextrose agar (PDA) ATCC® Medium 28: Emmons modification of Sabourauds agar |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.
GCCTGGCTTCGCGGCCGGCTGAAATATTTTTTTCACCCATGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCAAACCATAAACCTTTTTTTTATGCAGTTGCAATCAGCGTCAGTAAAAACAATGTAATTATTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAAGCTTTGCTTGGTGTTGGGCGTTTTTTGTCTCCCTCTTTCTGGGAGACTCGCCTTAAAACGATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACATATTTTGCGCTTTGTATCAGGAGAAAAGGACGGTAATCCATCAAGACTCTACATTTTTAACTTTTGACCTCGGATCAGGTAG D1D2 region of the 28S ribosomal RNA gene ATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGTAGGCAGGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTCTCTTCGGGGAGGCCTTATAGGGGAGACGACATACCACCAGCCTAGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCG |
| Name of Depositor： | BJ Steffenson |
| Isolation： | Barley leaves, Hordeum vulgare line I86-519-1, Prosper, Cass Co., ND |
| Cross References： | Nucleotide (GenBank) : JQ070161 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : JQ070090 ITS including 5.8S rRNA gene Nucleotide (GenBank) : AEIN00000000 Bipolaris sorokiniana ND90Pr, whole genome shotgun sequencing project |
| References： | Fetch TG Jr., Steffenson BJ. Identification of Cochliobolus sativus isolates expressing differential virulence on two-row barley genotypes from North Dakota. Can. J. Plant Pathol. 16: 202-206, 1994. Valjavec-Gratian M, Steffenson BJ. Genetics of virulence in Cochliobolus sativus and resistance in barley. Phytopathology 87: 1140-1143, 1997. Valjavec-Gratian M, Steffenson BJ. Pathotypes of Cochliobolus sativus on barley in North Dakota. Plant Dis. 81: 1275-1278, 1997. Zhong S, Steffenson BJ. Genetic and molecular characterization of mating type genes in Cochliobolus sativus. Mycologia 93: 852-863, 2001. Ohm RA, et al. Diverse lifestyles and strategies of plant pathogenesis encoded in the genomes of eighteen Dothideomycetes fungi. PLoS Pathog 8: e1003037, 2012. PubMed: 23236275 |