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| 产品名称： | pG4TH |
|---|---|
| 商品货号： | TS137935 |
| Designations： | pG4TH |
| GenBank Number： | J04758 |
| Species： | Mus musculus, mouse |
| Depositors： | D Goldman, DA Nielsen |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.728999972343445 Tissue: mastocytoma cell line P815 (ATCC TIB 64) Gene product: tryptophan hydroxylase (tryptophan 5-monooxygenase) Tph |
| Insert Size (kb)： | 1.729 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.6; BamHI--3.3, 1.3; HindIII--4.6; PstI--3.5, 1.1, EcoRI/BamHI--2.7, 1.2, 0.5 + smaller. The insert contains the following restriction sites (nt from the 5 end): BamHI--478; EcoRI--595; PstI--673; PvuII--1080. Detects genomic PstI fragments of the following sizes (kb): mouse--6.8, 4.3; Chinese hamster--5.6, 1.9, 0.9. Detects mRNA of approximately 1.9 kb (major) and 4.0 kb (minor) in P815 cells. Transcription from the SP6 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Stoll J, et al. Characterization and chromosomal mapping of cDNA encoding tryptophan hydroxylase from a mouse mastocytoma cell line. Genomics 7: 88-96, 1990. PubMed: 2110547 |