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| 产品名称： | pNRSac p Sac |
|---|---|
| 商品货号： | TS137947 |
| Designations： | pNRSac p Sac |
| Species： | Homo sapiens, human |
| Depositors： | RA Weinberg |
| Applications： | The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment. |
| Vector： | Construct size (kb): 9.0 |
| Insert： | DNA: genomic Insert lengths(kb): 7.0 Tissue: HL-60 cell line (promyelocytic leukemia) Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) NRAS Alleles: A1, A2 |
| Insert Size (kb)： | 7.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.5, 3.5; PvuII--4.2, 3.4, 1.0, 0.6. The insert can transform NIH 3T3 cells. The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment. Contains pBR322 (EcoRI/PvuII, 2.05 kb); SV40 ori (PvuII/HindIII, 344 bp); exon 1 (HindIII/HindIII); exon 2 (HindIII/EcoRI, 760 bp); exon 3/exon 4 (EcoRI/SacI, 5 kb), and SV40 polyadenylation signal (SacI/EcoRI). |
| References： | Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594 |