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| 产品名称： | pIP82 |
|---|---|
| 商品货号： | TS138082 |
| Designations： | pIP82 |
| GenBank Number： | X64810 |
| Species： | Homo sapiens, human |
| Depositors： | WJ Van De Ven |
| Applications： | produces protein proprotein convertase subtilisin/kexin type 1 neuroendocrine convertase 1 (pro-protein-convertase PC1) |
| Vector： | Construct size (kb): 6.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Vector end: XbaI Vector end: XbaI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PCSK1 Other gene symbols: NEC1 Genomic copy number: unique Gene name: proprotein convertase subtilisin/kexin type 1 Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q15-q21 Tissue: lung Type of DNA: cDNA Insert end: XbaI Insert end: XbaI Insert size (kb): 2.9 Cross references: DNA Seq. Acc.: X64810 Insert lengths(kb): 2.900000095367432 Tissue: lung Gene product: proprotein convertase subtilisin/kexin type 1(neuroendocrine convertase 1 (pro-protein-convertase PC1)) NEC1 Target Gene: proprotein convertase subtilisin/kexin type 1 |
| Insert Size (kb)： | 2.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.12; EcoRI--1.82; BamHI--1.97. The 3 XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present. Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record). |
| References： | Creemers JW, et al. Expression in human lung tumor cells of the proprotein processing enzyme PC1/PC3. Cloning and primary sequence of a 5 kb cDNA. FEBS Lett. 300: 82-88, 1992. PubMed: 1547893 Wim J Van De Ven, personal communication |