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| 产品名称： | NEB603, pMMfnuDIM302:20-6, pMMhaeIIIRM127-1 |
|---|---|
| 商品货号： | TS138089 |
| Designations： | NEB603, pMMfnuDIM302:20-6, pMMhaeIIIRM127-1 |
| GenBank Number： | M24625 |
| Species： | Fusobacterium nucleatum Knorr |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein |
| Insert： | DNA: genomic Gene product: restriction endonuclease HaeIII hsdM |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (50 mcg/ml) plus chloramphenicol (20 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pMMfnuDIM302:20-6 give the following sizes (kb): HindIII/XbaI--4.1, 1.7; EcoRI--6.0, 0.2; BamHI--6.2; HindIII--6.0. Restriction digest of pMMhaeIIIRM127-1 give the following sizes (kb):HindIII/XbaI--3.2, 2.7, 2.3, 2.2, 0.66; SacI--8.4, 2.7; EcoRI--3.8, 3.4, 1.5, 1.15, 0.96, 0.2; BamHI--11.25; HindIII--5.4, 2.7, 2.3, 0.68. This strain contains 2 plasmids. pMMfnuDIM302:20-6 is a 1.4 kb HindIII/XbaI fragment cloned into pACYC184 (cmlR) that expresses the modification methylase from F. nucleatum D. It was derived from pEVCfnuDIRM 102-1 (ATCC 40521) by deletion of internal HincII fragments and cloning into the the vector HindIII and XbaI sites. pMMhaeIIIRM127-1 is a 8.5 kb SacI fragment cloned into pUC19 (ampR) that expresses the restriction endonuclease HaeIII from H. aegyptius (ATCC 11116). The HaeIII methylase is expressed inadequately so the presence of pMMfnuDIM 302:20-6 is necessary to methylate host DNA and permit isolation of the HaeIII endonuclease. pMMhaeIIIRM127-1 is the predominant plasmid because of higher copy number. |
| References： | Wilson GG, Meda MM. Heterospecific modification as a means to clone restriction genes. US Patent 5,179,015 dated Jan 12 1993 Van Cott EM, Wilson GG. Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems. Gene 74: 55-59, 1988. PubMed: 3074019 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |