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| 产品名称： | BHK570 |
|---|---|
| 商品货号： | TS138139 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | kidney |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Used in producing a mammalian G protein coupled glutamate receptor U.S. Pat. 5,385,831.
The cells are useful as hosts for transfection studies. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | The BHK-570 cell line is a baby hamster kidney cell line that is thymidine kinase deficient (TK-) Ref   Horn IR, et al. Molecular analysis of ligant binding to the second cluster of complement-type repeats of the low density lipoprotein receptor-related protein. J. Biol. Chem. 272: 13608-13613, 1997. PubMed: 9153209. The cells are useful as hosts for transfection studies.
Used in producing a mammalian G protein coupled glutamate receptor U.S. Pat. 5,385,831. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | Univ. Washington, ZymoGenetics, Inc. |
| Deposited As： | hamster |
| U.S. Patent Number： | |
| References： | Mulvihill ER, et al. Method for producing a mammalian G protein copuled glutamate receptor. US Patent 5,385,831 dated Jan 31 1995 |