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微信小章| 产品名称： | c9189 9189 |
|---|---|
| 商品货号： | TS138149 |
| Designations： | c9189 9189 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Applications： | The ATCC encourages users of this material to communicate their results to us. |
| Vector： | Construct size (kb): 39.5 |
| Insert： | DNA: genomic Insert information: Insert size (kb): 33.1 Genexa0: HMR (mating-type cassette) DNAxa0: genomic DNA Contains complete coding sequencexa0: Unknown Sourcexa0: Saccharomyces cerevisiae AB972 (ATCC# 76269) Chromosomexa0: III xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Localizationxa0: III R, 97 cM from CEN Insert endsxa0: MboI Insert lengths(kb): 33.09999847412109 Gene product: mating-type cassette HMR |
| Insert Size (kb)： | 33.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of E. coli containing the cosmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 4.4, 4.1, 2.5, 2.3 (doublet), 2.1, 1.9 (doublet), 1.7, 1.6, 1.3 (doublet), 0.9 (doublet), 0.8, 0.6 (triplet), 0.5 (doublet), 0.35. Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.267, 0.937, 0.790, 0.485, 2.085, 1.906, 1.533, 0.429, 1.983, 1.295, 4.392, 4.113, 0.891, 0.609, 6.113, 2.390, 0.486, 2.593, 2.368, 1.630, 0.606, 0.571. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70206, 70863, 70896. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Wicksteed BL, et al. A physical comparison of chromosome III in six strains of Saccharomyces cerevisiae. Yeast 10: 39-57, 1994. PubMed: 8203151 |