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| 产品名称： | clone 13 |
|---|---|
| 商品货号： | TS138183 |
| Designations： | clone 13 |
| GenBank Number： | X68274 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TAX Other gene symbols: TAG1 Genomic copy number: unique Gene name: transiently-expressed axonal glycoprotein (axonin-1) Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 q32 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 4.5 Cross references: DNA Seq. Acc.: X68274 Insert lengths(kb): 4.5 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: axonal glycoprotein TAG-1( axonal glycoprotein TAG-1, axonin-1 cell adhesion molecule, transiently-expressed axonal glycoprotein, transiently-expressed axonal glycoprotein (axonin-1)) TAX |
| Insert Size (kb)： | 4.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.0, 0.6; HindIII/XbaI--4.5, 2.95; BamHI--4.3, 3.0, 0.2; PstI--3.15, 1.1 (doublet), 0.9, 0.6, 0.5; KpnI--3.3, 3.25, 1.0. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.26, 3.15; BglI--0.02, 1.15, 1.84, 2.26, 3.37, 3.86; EcoRI--0.57; KpnI--0.40, 1.38; PstI--0.57, 1.05, 2.12, 3.00, 3.16, 4.23, 4.25; PvuII--0.11, 0.44, 1.03, 1.91, 2.05, 2.60, 2.75, 3.32; SacI--2.35, 3.29, 3.55, 3.78; SmaI--0.36, 1.50, 2.46; XhoI--3.80. Contains the complete coding sequence, starting at the 5 end of the published sequence, with an additional T at position 2986, responsible for a frame shift and early stop codon. The GenBank record and reference show the corrected sequence. |
| References： | Hasler TH, et al. cDNA cloning, structural features, and eukaryotic expression of human TAG-1/axonin-1. Eur. J. Biochem. 211: 329-339, 1993. PubMed: 8425542 Kenwrick S, et al. Localization of the human TAX-1 gene to 1q32.1: a region implicated in microcephaly and Van der Woude syndrome. Hum. Mol. Genet. 2: 1461-1462, 1993. PubMed: 8242070 |