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| 产品名称： | sNF02.2 |
|---|---|
| 商品货号： | TS138204 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: lung |
| Cell Type： | Schwann Cell |
| Product Format： | frozen |
| Morphology： | Schwann cell-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neurofibromatosis type 1 (NF1) |
| Age： | 35 year old |
| Gender： | male |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The sNF02.2 was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin protein. |
| Clinical Data： | 35 years
male |
| Comments： | Western immunoblotting showed apparently full-length neurofibromin protein. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 twice weekly is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Culture medium,90%; DMSO,10%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 8,12 vWA: 16 |
| Population Doubling Time： | about 26 hours |
| Name of Depositor： | DF Muir, MR Wallace |
| Deposited As： | Homo sapiens |
| Passage History： | xadThe cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. xad |
| Year of Origin： | 2002 |
| References： | Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937 Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442 |