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| 产品名称： | pYC7 |
|---|---|
| 商品货号： | TS138308 |
| Designations： | pYC7 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.0600004196167000 Vector: pYC7 (plasmid) Promoters: Promoter truncated CYC1 promoter (no UAS element) Construction: pBR322 Marker(s):URA3,ampR Construct size (kb): 10.0600004196167 Features: marker(s): URA3 marker(s): ampR promoter: truncated CYC1 promoter (no UAS element) replicon: 2 micron ori replicon: pMB1 coding sequence: lacZ |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--10.3; HindIII--8.2, 2.25; BglII--10.3. This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites. DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity. The vector was constructed by William Timberlake. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chang YC, Timberlake WE. Identification of Aspergillus brlA responsive elements (BREs) by genetic selection in yeast. Genetics 133: 29-38, 1993. PubMed: 8417986 |
| Related Products： | component of:ATCC 87539 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried Escherichia coli containing the plasmid. |