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微信小章| 产品名称： | pTR1 |
|---|---|
| 商品货号： | TS138367 |
| Designations： | pTR1 |
| Species： | Rattus norvegicus, rat |
| Depositors： | LM Matrisian |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.700000047683716 Tissue: PyT21 cell line Gene product: matrix metalloproteinase 10( stromelysin 2, transin-2, stromelysin 2, stromelysin 2 (transin-2)) MMP3 |
| Insert Size (kb)： | 1.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (in nucleotides from the 5 end of the published sequence): PstI--151; HindIII--749; BamHI--1305. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; KpnI--6.0; HindIII--5.1, 0.9; PstI--4.3, 0.85 (doublet); BamHI--4.75, 1.25. pTR1 detects genomic EcoRI fragments of 6.0, 4.1, and 1.9 kb in rats and genomic EcoRI fragments of 12.0, 8.0, 3.5, and 2.7 kb in humans. The insert extends from nucleotides 91 to 1771 of the cDNA. The coding sequences extend from nucleotides 57 to 1482. The insert has the complete 3 untranslated region. A 525 bp PstI-KpnI fragment (nucleotides 155-680) of pTR1 detects a 7.9 kb genomic EcoRI fragment in rat cells which corresponds to the related gene, transin-2. pTR1 detects a 1.9 kb mRNA in both rat and mouse tumors. |
| References： | McDonnell SE, et al. Epidermal growth factor stimulation of stromelysin mRNA in rat fibroblasts requires induction of proto-oncogenes c-fos and c-jun and activation of protein kinase C. Mol. Cell. Biol. 10: 4284-4293, 1990. PubMed: 2115124 Matrisian LM, et al. Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts. EMBO J. 4: 1435-1440, 1985. PubMed: 3875482 Kerr LD, et al. Growth factors regulate transin gene expression by c-fos-dependent and c-fos-independent pathways. Science 242: 1424-1427, 1988. PubMed: 2462278 Kerr LD, et al. Transforming growth factor beta1 and cAMP inhibit transcription of epidermal growth factor- and oncogene-induced transin mRNA. J. Biol. Chem. 263: 16999-17005, 1988. PubMed: 2846555 Matrisian LM, et al. Differential expression of preproenkephalin and transin mRNAs following oncogenic transformation: evidence for two classes of oncogene induced genes. Oncogene Res. 2: 251-262, 1988. PubMed: 2835738 Matrisian LM, et al. The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than benign tomors. Proc. Natl. Acad. Sci. USA 83: 9413-9417, 1986. PubMed: 3540938 Matrisian LM, et al. Isolation of the oncogene and epidermal growth factor-induced transin gene: complex control in rat fibroblasts. Mol. Cell. Biol. 6: 1679-1686, 1986. PubMed: 2431284 Breathnach R, et al. Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene. Nucleic Acids Res. 15: 1139-1151, 1987. PubMed: 3547333 |