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| 产品名称： | pKLAf1IIRM520-4 |
|---|---|
| 商品货号： | TS138405 |
| Designations： | pKLAf1IIRM520-4 |
| Species： | Anabaena flos-aquae (Lyngbye) Brebisson, blue-green algae |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Vector： | Construct size (kb): 16.20 |
| Insert： | DNA: genomic Insert lengths(kb): 12.4 Gene product: modification methylase AflII Gene product: restriction endonuclease AflII |
| Insert Size (kb)： | 12.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.5, 3.4, 2.3; BamHI--17.0; HindIII--7.8, 4.4, 2.5, 1.1, 0.4; PstI--12.0, 4.7. The insert contains the following restriction sites (approximate kb from the 5 XhoII site): EcoRI--2.8, 5.0; HindIII--8.1, 10.6, 11.05, 12.05; NsiI--8.0; PstI--1.8; EcoRV--5.2; NcoI--11.8; SpeI--2.9, 5.5, 9.7; XhaII--2.1. Distributed in aliquots of 250 ng (10 ng/ul). |
| References： | Lunnen KD, Wilson GG. Method for producing the AflII restriction endonuclease and methylase. US Patent 5,030,569 dated Jul 9 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |