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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS138426 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | BW20767 |
| Application： | conjugation donor host host useful for transposon mutagenesis transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of Escherichia coli BW20767 |
| Storage Conditions： | Conditions: Store unopened frozen vial at -80°C. |
| Preceptrol®： | no |
| Genotype： | RP4-2tet::Mu-1kan::Tn7-integrant uidA(deltaMlu1)::pir+ recA1 creB510 leu-63 hsdR17 endA1 zbf-5 thi |
| Type Strain： | no |
| Comments： | Donor host for conjugative transfer of oriT-containing plasmids that utilize the R6Kgamma origin of replication. Also useful for transposon mutagenesis. Permits use of leu-63 (leucine-requiring) as a counterselectable marker. Phenotypes verified: Tn7 lac+ recA pir+ leu RP4tra+. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | BL Wanner |
| Special Collection： | NCRR Contract |
| References： | Barry L Wanner, personal communication |