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| 产品名称： | HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA |
|---|---|
| 商品货号： | TS138529 |
| Designations： | HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA |
| GenBank Number： | M10060 |
| Species： | human T-cell leukemia virus II |
| Depositors： | Irvin S. Y. Chen, Department of Medicine, University of California School of Medicine, Los Angeles, CA |
| Applications： | The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA. |
| Vector： | Vector name: pBR322 Intact vector size (in kb): 4.363 Type of vector: plasmid Vector ends: HindIII Features : marker(s): tetR, ampR replicon: pMB1 DNA Seq. Acc.: J01749 |
| Insert： | Genome: human T-cell leukemia virus II Contains complete coding sequence?: Y Type of DNA: genomic Insert ends: HindIII Insert size (kb): 10.1 DNA Seq. Acc.: M10060 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | 200 ng dried plasmid DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.0 (doublet), 0.92, 0.36; HindIII--10.5, 3.2; XbaI--13.5. The insert contains the following restriction sites (approximate kb from 5 end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4. The viral portion of the insert is 8.8kb. The viral portion of the insert is 8.8kb. The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA. The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells. Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb. |
| References： | Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323 Irvin S Chen, personal communication |