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| 产品名称： | HRRM2 |
|---|---|
| 商品货号： | TS138548 |
| Designations： | HRRM2 |
| GenBank Number： | X59618 |
| Species： | Homo sapiens, human |
| Depositors： | Y Yen |
| Vector： | Construct size (kb): 5.125 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCRII Intact vector size: 3.900 Type of vector: phagemid Vector end: TA site Vector end: TA site Cloning sites: TA cloning between EcoRI sites Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR, kanR promoter: lac, SP6, T7 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: RRM2 Gene name: ribonucleotide reductase M2 polypeptide Contains complete coding sequence?: Y Chromosome: 2; Localization: 2 p25-p24 Type of DNA: cDNA Insert 5 end: Modification: BamHI site Insert 3 end: Modification: HindIII site Insert size (kb): 1.17 Cross references: DNA Seq. Acc.: X59618 Nucleotides ~1~1169 of the insert correspond to nucleotides 195-1364 of X59618. Insert lengths(kb): 1.169999957084656 Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) small subunit( ribonucleotide reductase, small subunit, ribonucleotide reductase M2 polypeptide) RRM2 |
| Insert Size (kb)： | 1.170 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 1.2; HindIII--4.9; HindIII/EcoRI--3.9, 1.2; PstI--3.2, 1.1, 0.7; SalI--4.9. The insert contains the following restriction sites (approximate kb from the 5 end): SalI--0.08; PstI--0.68; EcoRI--1.14. Constructed by PCR using primers that generated a BamHI site upstream of the initiation codon (5 ATCCGGATCCACTATG 3) and a HindIII site downstream of the termination codon (5 TAAATAAGCTTAAGC 3). |
| References： | Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826 Yun Yen, personal communication |