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| 产品名称： | pAL49 |
|---|---|
| 商品货号： | TS138680 |
| Designations： | pAL49 |
| Depositors： | AC Leonard |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: pAL49 (plasmid) Construction: Escherichia coli oriC, pML21, Tn903 Marker(s):kanR,neoR Construct size (kb): 7.0 Features: marker(s): kanR, neoR replicon: oriC |
| Applications： | vector for other uses |
| Comments： | The plasmid minichromosome exhibits a moderate degree of instability and should be propagated in recA- strains grown in at least 50 mcg/ml kanamycin. A plasmid which replicates from the resident chromosome origin of replication, oriC. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Leonard AC, Helmstetter CE. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc. Natl. Acad. Sci. USA 83: 5101-5105, 1986. PubMed: 3523483 Leonard AC, et al. Involvement of DNA superhelicity of minichromosome maintenance in Escherichia coli. J. Bacteriol. 161: 687-695, 1985. PubMed: 2981821 |
| Shipped： | freeze-dried |