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| 产品名称： | SIM-A9 |
|---|---|
| 商品货号： | TS138750 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain; cerebral cortex |
| Cell Type： | microglia |
| Product Format： | frozen 1.0 mL |
| Morphology： | neuronal-like |
| Culture Properties： | mixed, adherent with some single cells in suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | postnatal 1 day |
| Strain： | C57BL/6 |
| Applications： | May serve as a model system for the investigation of microglial behavior in vitro in response to inflammatory stimuli, screening immunomodulating compounds, and brain malignancy research. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This cell line was derived from cortical tissues collected and pooled from mouse pups at postnatal day 1. The tissues were dissociated by trypsinization and plated. On day 14 the microglial cells were harvested by vigorously shaking the flasks and the plating out detached microglial cells. The plates were maintained for 2 weeks at which time an unexpected, extensive proliferation of microglial cells were observed. The cells were passaged 7 times over a 4 week period, and were determined to have become spontaneously immortalized. The cells were then plated out in a limited dilution procedure and colonies derived from wells observed to contain a single cell were expanded. A clone designated as “A-9” gave rise to this cell line. |
| Antigen Expression： | CD68+; ionizing calcium-binding adaptor molecule 1 (Iba1)+ xa0
Ref  Nagamoto-Combs K, et al. A novel cell line from spontaneously immortalized murine microglia. J. Neurosci. Methods 233: 187-198, 2014. PubMed: 24975292 |
| Comments： | These spontaneously immortalized micgoglial cells exhibit phagocytic activity upon liposaccharide stimulation and TNFα cytokine secretion upon β-amyloid exposure. These cells may be used in place of primary microglial cultures, which require a lengthy and costly preparation procedure. |
| Complete Growth Medium： | The base medium for this cell line is DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: every 2 to 3 days |
| Cryopreservation： | Complete growth medium, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37°C |
| Volume： | 1.0 mL |
| Name of Depositor： | K Nagamoto-Combs |
| References： | Nagamoto-Combs K, et al. A novel cell line from spontaneously immortalized murine microglia. J. Neurosci. Methods 233: 187-198, 2014. PubMed: 24975292 |