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| 产品名称： | lambda7 |
|---|---|
| 商品货号： | TS138803 |
| Designations： | lambda7 |
| GenBank Number： | L10641 |
| Species： | Homo sapiens, human |
| Depositors： | A Dugaiczyk |
| Vector： | Construct size (kb): 50.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 19.1 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~1-19100 of the insert correspond to nucleotides ~29850-49180 of L10641. Insert lengths(kb): 19.10000038146973 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC |
| Insert Size (kb)： | 19.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 7.2, 4.3, 3.3, 2.7, 1.6; HindIII--10.5, 9.55, 8.8, 5.75, 4.35, 4.1, 1.5, 1.4, 0.9, 0.6, 0.3; BamHI--13, 8, 6.6, 6.2, 3.9 (doublet), 3, 2.3, 1.5, 0.5; Restriction digests of the clone give the following sizes (kb): BglII--14, 9.2, 5.8, 5, 4.9, 2.7, 2.2, 1.9, 1.8, 1.35, 1, 0.65, 0.35; SstI--18, 11, 4.6, 4.2, 3.85, 2.4, 1.95, 1.65, 1.2. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.6, 4.3, 8.6, 11.9; HindIII--0.4, 1.5, 2.9, 3.8, 4.2, 13.75, 18.1; SacI--14.6, 18.8. Begins in intron 6 and extends into the 3 flanking region. ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb. TS138803/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb. Contains an Alu repeats, and part of a THE repeat. |
| References： | Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650 |