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| 产品名称： | PRL-Rs3 probe |
|---|---|
| 商品货号： | TS138863 |
| Designations： | PRL-Rs3 probe |
| GenBank Number： | M22957 |
| Species： | Mus musculus, mouse |
| Depositors： | DI Linzer |
| Vector： | Construct size (kb): 3.09500002861023 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.2259999960660934 Tissue: liver (pregnant) Gene product: prolactin receptor Prlr |
| Insert Size (kb)： | 0.226 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaII--2.8, 0.25; BamHI--3.1; BglI--3.1; DraI--2.4, 0.74; EcoRI--2.8, 0.28; PstI--3.1. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.03, HaeIII--0.15. A unique sequence probe corresponding to nt 1066-1291 of the published sequence for form 3 of the receptor. Transcription from the SP6 promoter gives the sense strand (linearize with HindIII. Transcription from the T7 promoter gives the anisense strand (linearze with BamHI). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Clarke DL, Linzer DI. Changes in prolactin receptor expression during pregnancy in the mouse ovary. Endocrinology 133: 224-232, 1993. PubMed: 8319571 Davis JA, Linzer DI. Expression of multiple forms of the liver prolactin receptor in mouse liver. Mol. Endocrinol. 3: 674-680, 1989. PubMed: 2725531 Daniel I Linzer, personal communication |