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| 产品名称： | pVEX1211 EKA607 |
|---|---|
| 商品货号： | TS138864 |
| Designations： | pVEX1211 EKA607 |
| Depositors： | DH Figurski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli BR2943 |
| Vector Information： | Size (kb): 3.5000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 3.500 Type of vector: plasmid Cloning sites: Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI SacI XhoI BglI HindIII Construction: pVEX1191 Host range: Escherichia coli Features (with orientation and position when available): replicon: P1 restriction site: PstI marker(s): strR, spcR restriction site: NotI MCS: HindIII...EcoRI, -> restriction site: NotI other: loxP, -> restriction site: PstI Vector: pVEX1211 (plasmid) Construction: pVEX1191 Marker(s):spcR,strR Construct size (kb): 3.5 Features: marker(s): strR, spcR other: loxP replicon: P1 MCS: HindIII...EcoRI restriction site: NotI restriction site: PstI |
| Applications： | host modification vector for other uses vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; PstI--2.1, 1.1, 0.35. To be used with pVEX2211 or pVEX2212 (ATCC 77422, 77423). The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when spectinomycin selection is imposed in a host not expressing repA. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates pVEX1211(pVEX1212) and pVEX2211(pVEX2212) provide two loxP sites for site-specific recombination that deletes the intervening target sequence. Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. Differs from pVEX1212 (ATCC 77421) only in the orientation of the polylinker. |
| Media： | ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |