微信小陈
微信小章| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS138882 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | Jade 6 |
| Application： | produces protein 1-aminocyclopropane-1-carboxylate deaminase |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
| Preceptrol®： | no |
| Type Strain： | no |
| Comments： | Growth in M9 plus 0.05 mM ACC or co-expression of ACC synthase complement the Ile auotrophy. E. coli host expressing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminse from the recA promoter. Strain was derived from JHM-544 (ATCC 12911), an Ile auxotroph, by chromosomal integration of the ACC deaminase using cre-lox-mediated recombination. |
| Medium： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | A Theologis |
| Special Collection： | NCRR Contract |
| References： | Tarun AS, et al. Random Mutagenesis of 1-aminocyclopropane-1-carboxylate synthase: a key enzyme in ethylene biosynthesis. Proc. Natl. Acad. Sci. USA 95: 9796-9801, 1998. PubMed: 9707555 Klee HJ, et al. Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell 3: 1187-1193, 1991. PubMed: 1821764 Hasan N, et al. Escherichia coli genome targeting, I. Cre-lox-mediated in vitro generation of ori- plasmids and their in vivo chromosomal integration and retrieval. Gene 150: 51-56, 1994. PubMed: 7959062 |