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| 产品名称： | RA-1 |
|---|---|
| 商品货号： | TS138949 |
| Designations： | RA-1 |
| GenBank Number： | D16217 |
| Species： | Homo sapiens, human |
| Depositors： | H Menard |
| Applications： | produces protein calpastatin |
| Vector： | Construct size (kb): 5.919000148773193 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEX-4T-1 Intact vector size: 4.969 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SmaI SalI XhoI NotI Polylinker sites: EcoRI SmaI SalI XhoI NotI Other unique sites: PstI mLuI BstEII ApaI BssHII EcoRV NarI BamHI Construction: pGEX-2T Host range: Escherichia coli Features (with orientation and position when available): other: thrombin protease cleavage site MCS: EcoRI/.../NotI marker(s): ampR, -> replicon: pMB1 repressor gene: lacI, -> promoter: tac, -> coding sequence: S. japonicum glutathione S-transferase Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CAST Gene name: calpastatin Contains complete coding sequence?: N Chromosome: 5; Localization: 5 q15-q21 Tissue: placenta Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 0.95 Cross references: DNA Seq. Acc.: D16217 Nucleotides 1-950 of the insert correspond to nucleotides 1435-2384 of D16217. Insert lengths(kb): 0.949999988079071 Tissue: placenta Gene product: calpastatin CAST Target Gene: calpastatin |
| Insert Size (kb)： | 0.950 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 1.0; BamHI--5.9; PstI--4.6, 1.35; HindIII--5.4, 0.5; EcoRI/PstI--4.2, 1.0, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.13, 0.54; PstI--0.66. This clone expresses a fusion protein of GST and calpastatin (domains 3 and 4 only). This truncated product also differs from calpastatin by a substitution (G->A) at nt 1937 of D16217, resulting in a glycine to glutamate substitution (G592E). |
| References： | Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496 |