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| 产品名称： | Crypthecodinium cohnii (Seligo) Javornicky |
|---|---|
| 商品货号： | TS138976 |
| Strain Designations： | GP1 |
| Application： | Biofuel production |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Zostera sp., Greenport, Long Island, NY |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Reproductively isolated from the WH group. Indistinguishable from strains of major sibling species WH based upon zymogram patterns of soluble enzymes and SSU rRNA sequences. Minor Sibling Species Gp |
| Medium： | ATCC® Medium 460: A2E6 medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss. |
| Subcultivation： | Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss. |
| Name of Depositor： | CA Beam, M Himes |
| References： | Beam CA, Himes M. Electrophoretic characterization of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 34: 204-217, 1987. |