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| 产品名称： | pMAP3 |
|---|---|
| 商品货号： | TS138989 |
| Designations： | pMAP3 |
| GenBank Number： | D00117 |
| Species： | Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors： | Monsanto Co., FJ Perlak, Monsanto Co. |
| Applications： | produces protein crystal protein (delta endotoxin) |
| Vector： | Construct size (kb): 10.80000019073486 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC8 Intact vector size: 2.700 Type of vector: plasmid Vector end: BamHI Vector end: PstI Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Construction: pBR322, Escherichia coli lacZalpha, M13mp8 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: Y Strain: HD-1 Type of DNA: plasmid Insert 5 end: BamHI Insert 3 end: PstI Insert size (kb): 8.1 Cross references: DNA Seq. Acc.: D00117 Insert lengths(kb): 8.100000381469727 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 8.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/PstI--8.1, 2.7; EcoRI--5.2, 1.9 (doublet), 0.7, 0.6; HindIII--8.0, 1.5, 1.1. The following microorganisms are suitable for recombination and plant-colonization: Pseudomonas, Agrobacterium, Rhizobium, Erwinia, Azotobacter, Azospirillum, Klebsiella, Alcaligenes and Flavobacterium. The coding sequence within the insert can be integrated into the chromosome of one of several plant-colonizing microorganisms to protect the colonized plant against insect pests. The complete coding sequence for the toxin is contained within an internal 4.6 kb HpaI-PstI fragment. |
| References： | Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |