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| 产品名称： | pG143/T2 |
|---|---|
| 商品货号： | TS139036 |
| Designations： | pG143/T2 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 17.5 |
| Insert： | DNA: genomic Insert lengths(kb): 9.300000190734863 Gene product: transcription activation factor HAP3 HAP3 |
| Insert Size (kb)： | 9.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (approximate kb): EcoRI- 2.2- XbaI- 0.6- SstI- 0.7- EcoRI- 0.9- BamHI- 0.25- SphI- 0.55- SstI- 0.1- SphI- 0.7- XbaI. Restriction digests of the clone give the following sizes (kb): 12.0, 4.65, 1.0; BamHI--17.5; SmaI/SalI--7.6 (doublet), 2.25; KpnI--uncut. The HAP3 gene is included in a 1.6 kb BamHI/XbaI fragment. The HAP3 locus encodes 2 overlapping transcripts of 0.57 kb and 4.0 kb read from opposite strands. The smaller mRNA, encoding a protein of 144 aa, directs the synthesis of a protein required for UAS2 activity. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Hahn S, et al. The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts. Mol. Cell. Biol. 8: 655-663, 1988. PubMed: 2832732 Alexander Tzagoloff, personal communication |