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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS139056 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | XD99 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa/MATalpha ade2-1/ade2-1 his5-2/his5-2 lys1-1/lys1-1 can1-100/can1-100 +/ura2-1 SUP7/+ +/ilv3-1 SUP4/+ trp1/+ (M. Dixon, personal communication) |
| Preceptrol®： | no |
| Ploidy： | diploid |
| Comments： | Strains for detection of mutations, recombination, and aneuploidy (BG1) XD99 was constructed to screen for mitotic chromosome loss events. can1-100 is an ochre-suppressible mutation for canavanine resistance. As long as this strain carries both SUP4 and SUP7, it will be canavanine-sensitive. If it loses the copy of chromosome X that carries these two ochre suppressors, it will become canavanine-resistant. At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| Medium： | ATCC® Medium 1069: YPAD medium |
| Growth Conditions： | Temperature: 25.0°C Atmosphere: Typical aerobic |
| Name of Depositor： | YGSC |
| Special Collection： | Yeast Genetic Stock Center |
| Chain of Custody： | ATCC < |
| Isolation： | XD99 was constructed to screen for mitotic chromosome loss events. can1-100 is an ochre-suppressible mutation for canavanine resistance. As long as this strain carries both SUP4 and SUP7, it will be canavanine-sensitive. If it loses the copy of chromosome X that carries these two ochre suppressors, it will become canavanine-resistant. At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| References： | M. Dixon, personal communication M. Dixon, personal communication |