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| 产品名称： | c8083 |
|---|---|
| 商品货号： | TS139064 |
| Designations： | c8083 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 50.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: TOP3 Gene name: topoisomerase (DNA) III Contains complete coding sequence?: Y Chromosome: XII Genome: Saccharomyces cerevisiae Gene symbol: EST1 Gene name: telomere elongation protein (probable telomerase) Contains complete coding sequence?: Y Chromosome: XII Genome: Saccharomyces cerevisiae Gene symbol: CDC42 Gene name: cell division cycle 42 (GTP-binding protein, 25kD) Contains complete coding sequence?: Y Chromosome: XII Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 43.5 Cross references: Insert lengths(kb): 43.5 Gene product: cell division cycle 42 (GTP-binding protein, 25kD) EST1 |
| Insert Size (kb)： | 43.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen, uncharacterized |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |