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| 产品名称： | pSP64-f1- |
|---|---|
| 商品货号： | TS139065 |
| Designations： | pSP64-f1- |
| Depositors： | DA Mead |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5000000000000000 Vector: pSP64-f1- (phagemid) Promoters: Promoter SP6 Construction: bacteriophage f1, pSP64 Marker(s):ampR Construct size (kb): 3.5 Features: marker(s): ampR promoter: SP6 replicon: pMB1, f1 |
| Applications： | vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA |
| Comments： | M9 medium is required to retain f1. This plasmid vector can be used for in vitro RNA synthesis from the SP6 promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin M9 |
| References： | Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a stretched preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795 |
| Shipped： | freeze-dried |