产品名称: | Rubella virus |
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商品货号: | TS139071 |
Classification: | Togaviridae, Rubivirus |
Agent: | Rubella virus |
Strain: | RA27/3 |
Common Name: | Rubella virus |
Applications: | Respiratory research |
Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format: | frozen |
Storage Conditions: | -70°C or colder |
Comments: | Attenuated for humans. Hemagglutinates baby chicken or trypsinized human O type cells. |
Effect on Host: | No distinct CPE in MRC-5, but there is some general deterioration in MRC-5. LLC-MK2 has more distinct rounding CPE at high titer. |
Recommended Host: | MRC-5 (ATCC® CCL-171™x99) |
Growth Conditions: | Incubation: 12-14 days at 30°C, a 5% CO2 in air atmosphere is recommended. Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent). |
Name of Depositor: | SA Plotkin |
Source: | Human fetus with congenital rubella |
References: | Plotkin SA, et al. Studies of immunization with living rubella virus. Trials in children with a strain cultured from an aborted fetus. Am. J. Dis. Child. 110: 381-389, 1965. PubMed: 5834207 Plotkin SA, et al. Attenuation of RA 27/3 rubella virus in WI-38 human diploid cells. Am. J. Dis. Child. 118: 178-185, 1969. PubMed: 5794813 |