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| 产品名称： | pPA8 |
|---|---|
| 商品货号： | TS139081 |
| Designations： | pPA8 |
| GenBank Number： | X07653 |
| Species： | Barley yellow dwarf luteovirus |
| Depositors： | WA Miller |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.700000047683716 Gene product: coat protein |
| Insert Size (kb)： | 1.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert includes the following restriction sites (with the PstI site adjacent to the HindIII site of the polylinker = 1): AvaI--151; BamHI--187; PvuII--620; AvaI--711; KpnI--870; EcoRI--876; AccI--1563; EcoRI--1683. Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.7; EcoRI--2.9, 0.8, 0.7; HindIII--4.4. The insert is oriented with the 5 side (3308) closer to the EcoRI site of the polylinker. The insert includes nucleotides 3308 through 5011, encoding part of the coat protein and the overlapping 17K ORF, all of the 50K ORF, and part of the 6.7K ORF. |
| Classification： | Luteovirus |
| References： | Miller WA, et al. Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Res. 16: 6097-6111, 1988. PubMed: 3399386 W A Miller, personal communication |